sterility:sterile-filtered.
form:liquid.
technique(s):cell culture | mammalian: suitable.
components:HEPES: 7.5 mM;NaHCO3: 1.6 g/L;glucose: 5.6 g/L;L-glutamine: no;phenol red: yes.
Application:EX-CELL CD CHO is a chemically defined, animal-component free, serum-free medium developed for the long-term growth of Chinese Hamster Ovary (CHO) cells and expression of antibodies or protein products in suspension cell culture. CHO suspension cultures can be subcultured directly into EX-CELL CD CHO from serum-supplemented or serum-free medium with little or no adaptation. EX-CELL CD CHO is formulated without hypoxanthine, thymidine or L-glutamine, making it an appropriate medium for selection systems such as DHFR- and Glutamine Synthetase (GS System). For applications that do not require the selective pressure of a hypoxanthine/thymidine (HT)-deficient medium, we recommend the use of EX-CELL CD CHO with hypoxanthine and thymidine (Catalog No. 14361C).